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The combined extracts were filtrated and concentrated to remove ethanol.
The mixture was then concentrated to remove the THF and then treated with distilled water.
The mixture was then concentrated to remove the THF and then washed with NaHCO3 (2 N, 30 mL).
Reaction mixture was washed with 1 M HCl, 10%% bicarbonate solution followed by water and brine, dried over sodium sulfate, and concentrated to remove solvent under reduced pressure.
The solution was concentrated to remove the ethanol.
Crude oligosaccharides were concentrated to remove the organic solvent and additives prior to SEC fractionation.
Similar(50)
It was concentrated initially to remove the solvent and then concentrated under vacuum to remove water at temperature less than 60°C.
The samples were then concentrated by a speed-vac to dry, cleaved with the ICAT tm cleaving reagent containing 95% TFA and concentrated again to remove the cleaving reagent.
This was filtered and concentrated to a small volume to remove the entire methanol using rotary evaporator.
The filtrate obtained was concentrated to a small volume to remove the entire methanol using rotary evaporator.
Amplification products were removed, concentrated to approximately 20 μL and separated by electrophoresis on 1.4% agarose gels (stained with ethidium bromide, 10 mg/mL stock) in 1X Tris-borate-EDTA (TBE) buffer at 60 V for 14 cm, and visualized under UV light (14 ).
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