Sentence examples for concentrated to appropriate from inspiring English sources

Collected samples were homogenized in 10 mM Tris-HCl, pH 8 with ice-cold Dounce tissue grinder (Wheaton, MillVille, NJ) and centrifuged twice at 12,000×g for 10 min. Supernatant was concentrated to appropriate volume with vacuum concentrator, separated with 15, 18 and 20% native PAGE and subsequently stained with Coomassie Brilliant Blue R-250 (CBB) (Bio-Rad, Hercules, CA).

An aliquot of supernatant was concentrated to the appropriate volume with vacuum concentrator and analyzed by 15% native-PAGE.

The pooled protein fractions were concentrated to 0.5 ml using an appropriate molecular-mass cut-off spin concentrator (Millipore) and applied to the SEC column using a 1 ml sample loop.

The appropriate fractions were concentrated to ∼20 40 mg/mL by ultrafiltration (Amicon Ultra-4 membrane, 10 000 NMWL).

Once the A280 returned to a baseline reading, the desired protein was eluted by adding 10 mL of reduced glutathione (10 mM, freshly prepared) in Tris HCl (50 mM, pH 8.0), and then the appropriate fractions were concentrated to ∼20 40 mg/mL by ultrafiltration (Amicon Ultra-4 membrane, 10 000 NMWL).

The His-tagged PH-Akt1 was concentrated to ca. 20 mg/ml using appropriate Amicon centrifugal filter units (Millipore).

The PCB concentrates was digested with aqua regia, cooled, and then diluted to appropriate concentration range using deionized water.

To address the costly rise in antidepressant prescribing, future research and guidance needs to concentrate on appropriate long term prescribing for depression and regular review of medication.

Above all, you'll need to concentrate on appropriate proportions throughout the sculpting of the bear, so it can be very helpful to have images of real bears in front of you––print a few from the internet or borrow a book on bears from your local library.

Cleaved Pikp-HMA was purified from the digest using a Ni2+-NTA column, collecting the eluate, followed by dialysis in buffer B and was then concentrated as appropriate.

Furthermore, researchers should concentrate on establishing physician-friendly rules for assigning patients to appropriate phenotypes in daily practice.