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Fractions containing CaRF as determined by Western analysis were pooled and concentrated to a final concentration of 100ng/uL.
Each decoction was then concentrated to a final concentration of 1 crude drug gram per milliliter.
The sample was concentrated to a final concentration of approximately 0.1 mM.
The decoction was filtered and then concentrated to a final concentration of 1 g/mL (crude drug/decoction).
SolAC fractions were pooled and concentrated to a final concentration of ∼10 mg mL−1 using a vivaspin 2 centrifugal concentrator (HY membrane).
Purified proteins were dialyzed against the buffer containing 50 mM Na2HPO4 (pH7.4) and 20 mM NaCl and concentrated to a final concentration of 0.5 1.5 mM.
Fractions containing the Fc sFcγRIIIa complex were concentrated to a total protein concentration of 20 mg/mL and used for crystallization.
Purified N-CLCA1 was concentrated to a final volume of 300 µl in a centrifuge concentrator and protein concentration was calculated from absorbance at 280 nm.
The protein was then concentrated to a range between 10 and 114 mg/mL using a centrifugal concentrator.
Finally, samples were concentrated to a final volume of 1 mL with a Vivaspin6, centrifugal concentrator, as described above.
By this process the pollutants are concentrated to a level such that straightforward analysis and monitoring can take place.
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