Sentence examples for concentrated template from inspiring English sources

In addition, these targets could only be detected from bacteria grown in vitro when 100-fold concentrated template RNA was analyzed, emphasizing the very low levels of these transcripts.

Create a contract template.

After PCR, these products were pooled, digested with Dpn1 for 4 hr at 37°C to remove vector template, and concentrated by isopropanol precipitation.

Analytes were first extracted from the samples using methanol/NaOH by an ultrasound-assisted method, and then concentrated on a Carbowax-templated resin (CW-TPR) SPME fiber.

Bayesian epistemologists have attempted to formalise extant explanations of transmissivity and non-transmissivity of justification; they have concentrated on the information-dependence template.

All techniques were converted onto electronic templates concentrating on addiction and lifestyle elements.

All techniques were converted into electronic templates concentrating on domain elements.

qPCR reactions, composed of cDNA template, Universal TaqMan MasterMix (2× concentrated, Life Technologies), TaqMan assay (20× concentrated, Life Technologies) and H2O, were performed with the 7500 Fast Real-Time PCR System (LiFast Real-Times).

After second-strand synthesis is completed, the template is then transcribed by a highly concentrated T7 polymerase, resulting in a ∼2000-fold ∼2000-foldion of amplification that can be used fof hybridisantisenselysis (Phillips and EbeRNAne, 1996).

The amplified cassette should be gel purified to remove any template from the PCR reaction and if necessary concentrated for electroporation.

Therefore, for Ranunculus macranthus the NaCl method [ 68] was used and yielded concentrated, sufficiently pure cpDNA and was used as the sequencing template.