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They were lost to contamination however two out of these three specimens had smear positive results from their processed concentrated specimens.
Concentrated specimens were stained for the presence of acid-fast bacilli (AFB) according to the Ziehl-Nielsen method [31].
Concentrated specimens were cultured in 3% Ogawa medium and observed weekly for 9 weeks after inoculation.
Concentrated specimens were then evaluated using both Kinyoun staining and auramine-rhodamine fluorochrome staining with fluorescence microscopy.
Concentrated specimens were cultured in MGIT tubes (Becton-Dickinson and Co.; Sparks, MD, USA) as well as in 3% Ogawa medium and observed weekly for 6 or 9 weeks after inoculation, respectively.
The study sites laboratories were allowed to miss detection of the low concentrated specimens.
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To address this issue, we now concentrate specimens, particularly from lymph nodes of patients with CGD, by centrifugation, place the specimens on BCYE and Middlebrook 7H11 agar, and incubate cultures for a minimum of 2 weeks.
After multiple RNA extractions were conducted and the RNA was concentrated, this specimen was found to be positive for the neuraminidase 1 (N1) gene by real-time PCR.
In addition, 9/11 (82%) concentrated nasal wash specimens from HRV infected children were positive in the 3Cpro test.
However, the acute temperature and cooling rate changes are mainly concentrated on the specimen surface region about 1/3 of the sample thickness, while these changes are performed in a slow and gentle manner at the core region of specimen.
However, it should be emphasized that the acute temperature and cooling rate changes during DCT are mainly concentrated on the specimen surface region about 1/3 of the sample thickness.
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