TES samples were filtered with a Millex filter unit (0.2 μm pore size), concentrated using an ultrafiltration device (Amicon), and stored at −20°C until needed.
Supernatants containing pseudo-typed particles were collected, filtered through a PVDF filter (0.45 μm pore size), and concentrated by centrifugation at 3800 × g for 30 min at 4°C in an Amicon Ultra-15 centrifugal filter device (100 K, Merck Millipore) according to the manufacturer's instructions.
The as-synthesized mesoporous SrCO3 microspheres had a specific surface area of 56.3 m2 g− 1 and a narrow pore size distribution concentrating on 2 20 nm, with an average pore diameter of 9.45 nm.
The MB-derived ACs have as large specific surface area as up to 2151.92 m2g−1 and the pore size distribution concentrates on 2 5 nm.
The dust is nonuniform in our galaxy and mainly concentrated with sizes of tens of pc.
Media were concentrated using Vivaspin column (pore size 1 × 10; Vivascience, Munich, Germany).
The culture supernatants were collected every 24 h for 3 days, filtered through a 0.45 um pore size filter, and concentrated twice via ultracentrifugation at 50,000 xg at 20°C for 120 min.
Therefore, they concentrate on size fractions ≤250 μm due to the higher probability of these particles' sizes to be incorporated as stable aggregates (e.g., Veitch and Woods 2001; Textor et al. 2006a, b; Durant et al. 2008; Costa et al. 2010; Folch et al. 2010).
"Concentrating on size alone is why the three big gorillas have gotten into such trouble," he says.
All size groups showed best hearing abilities in frequency ranges where main energies of stridulation sounds were concentrated, and all size groups were able to detect conspecific sounds.
