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This year brings the 39th annual festival, which has become a tradition of its own, nearly as central to the New Orleans calendar as Mardi Gras and, for music-loving visitors, a much more convenient and concentrated sampling of New Orleans and Louisiana music.
This assessment is probably erroneous, however, because a concentrated sampling effort has been lacking, and too few samples of these taxa have been studied.
However, because this was not the focus of our analysis, our sampling strategy is not optimal for this question, and the robustness of this pattern needs to be examined with more concentrated sampling in those lineages.
These results from a rather well studied habitat (Europe's freshwater) suggest that it is likely that concentrated sampling of marine fish in poorly known areas of Europe's coastal and deep-sea zones, and in Arctic areas in particular, may result in comparable changes in our understanding of fish biodiversity and perhaps in latitudinal trends in that diversity.
More concentrated sampling within certain genera has allowed further assessment of geographic patterns within specific deep-sea lineages.
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The protein concentration of the concentrated samples was determined by the Lowry method.
An increasing concentration of acetonitrile elutes the concentrated sample and further separation was achieved onto the analytical Zorbax 300SB-C18 reversed-phase column.
Peak trapping of OSCS provided a route to the on-line concentration of the contaminant, presenting a more concentrated sample for H NMR identification and structural characterization.
For sewage water, 100 mL were concentrated by a Molecular Weight Cutoff (MWCO) 100,000 Da Vivaspin20 centrifugal concentrator (Sartorius, Madrid, Spain) to a 5-mL concentrated sample.
The remaining concentrated sample was subjected to ELISA.
The concentrated samples were filtered through 0.45 μm membrane filters.
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