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Anyway the optical properties we measured on more lightly concentrated samples can be easily scaled to higher concentrations if needed.
Such preparation is necessary because the 454 is sensitive to PCR concentration and a run may become easily biased by highly concentrated samples.
Then the conductivity increased again about one order at 60 mol% addition and reached a constant value for highly concentrated samples.
Lavage recovery was similar in all animals and the data shown are those from the concentrated samples.
Concentrated samples were taken in 1 µl MALDI matrix (α-cyano-hydroxycinnamic acid in acetonitrile/0.1% TFA, 0.7∶0.3).
Concentrated samples were diluted with 1 mL of 100 mM CaCl2 in 20 mM TRIS-HCl buffer at pH 7.4.
Concentrated samples were loaded on the top of a 3.4 ml sucrose gradient in Beckman centrifuge tubes, Cat. No. 349622.
Concentrated samples need to be diluted, while samples with low O. cf. ovata abundances need to be counted in higher volumes or even in multiple sub-samples.
Concentrated samples (10 µg) were subjected to non-reducing SDS-PAGE as described in the previous study [50] using gels co-polymerized with 0.1% gelatin or 0.1% casein and 10 µg/mL plasminogen (Sigma-Aldrich).
Concentrated samples were recovered by withdrawing with a pipettor.
Concentrated samples were then stored for 10 days.
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