Sentence examples for concentrated passed from inspiring English sources

Then, the solution was concentrated, passed through an amylose resin chromatography column pre-equilibrated with 25 mM Tris, pH 7.5, 150 mM NaCl and 0.5 mM EDTA, and subsequently purified on a Hiload 16/600 Superdex 75 column (GE Healthcare, USA), washed with the buffer containing 25 mM NaH2PO4, pH 6.80 and 50 mM NaCl.

The non-retarded fraction was concentrated, passed through a CL-4B Sepharose column and the major peak, composed of Glu, freeze-dried.

The filtrate was concentrated and passed over a short plug of SiO2 to give a syrup (9.9 g, 18 %) with peracetates (pyranoses α/β 49 32 + 18%% α-furanoside) as the main components.

When the fish of Clear Lake were also analyzed, the picture began to take form: The poison had been picked up by the smallest organisms, concentrated, and passed on to the larger ones, which concentrated it further….

When the fish of Clear Lake were also analyzed, the picture began to take form: The poison had been picked up by the smallest organisms, concentrated, and passed on to the larger ones, which concentrated it further.

The PRMT5 MEP50 complex was eluted with 0.1 mg/ml of FLAG peptide (Sigma; F3290) in the same buffer, concentrated, and passed over a size-exclusion chromatography column (16/60 S300; GE LifeSciences) equilibrated in a buffer containing 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10% (vol/vol) glycerol, and 2 mM DTT.

The protein was concentrated and passed through a Superdex 75 column (GE Healthcare), equilibrated in 10 mM HEPES pH 6.9, 150 mM NaCl, 5 mM N octyl D glucoside (OG) (Anatrace Inc., Maumee, OH, USA) and 3 mM tris(2 carboxyethyl phosphine (TCEP).

The flow through was collected, concentrated and passed through a size-exclusion chromatography column (HR 30/100 GL, Superdex 75 prep grade, GE Healthcare) equilibrated with buffer C to remove free biotin.

The flow through was collected, concentrated and passed through a size-exclusion chromatography column (HR 30/100 GL, Superdex 75, GE Healthcare) equilibrated with buffer C {10 mM Tris/HCl (pH 7.5), 10 mM NaCl and 0.5 mM TCEP [tris- 2-carboxyethyl phosphine)]}.

The nematodes were collected from the water, then concentrated by passing through a 20-μm sieve.

For western blot analysis, the MLF CMs were concentrated by passing through a 3-kDa cut-off filtration membrane (Vivaspin 20, Sartorius).