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A concentrated master mix was made containing equimolar concentrations of the targets, and aliquoted, with the aliquots frozen at −20C.
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Since then, social scientists have found evidence supporting Hayes's theory in studies of chess, sports, and more; research continues to show that it takes innate talent and about a decade of concentrated effort to master a craft.
Each reaction contained half of the 2× concentrated Hotstart Q-master mix (ABgene), 1 pmol forward and reverse primer (see Table S2 for sequences), 10 pmol of the FAM-tagged universal Z probe (Intergen), and the cDNA.
PCR master mix concentrations.
They were masters of building up concentrated local support and turning it into council seat wins.
Although Mr. Ishii considered Mr. Naruse to be his mentor and master, it is hard to find any trace of Mr. Naruse's concentrated, claustrophobic use of space and deliberate, ritualized movement in "Horrors of Malformed Men".
"When I think about someone being a master, I would think more concentrated, and more focused, and one direction.
In this program, the trainee spends additional year(s) pursuing a master's or PhD degree, or concentrated time training in a lab or with a clinical researcher.
These interests spread out to include national groups, but also became more concentrated around industrial history, leading Christine to take a master's at Sheffield University in 1992, with a dissertation Millwrights in Sheffield and South Yorkshire 1550-1900.
In order to identify the master sex gene in rainbow trout a concentrated sequencing effort of the sex chromosome is needed.
Over the years Mr. Frank's repertory has been conservative, concentrated with occasional exceptions on the European Classical and Romantic masters.
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