We are not aware of a secreted molecule of importance that could have been removed by the method we have used to produce conditioned medium and concentrate it for in vivo use.
Purified RNA was then ethanol precipitated to concentrate it for 5′-end labeling using a 5′ EndTag kit and fluorescein maleimide (Vector Labs, Burlingame, CA) to enable gel visualization in subsequent steps; the RNA was then purified again using RNA Clean & Concentrator columns.
The wide green band containing PSI was collected and loaded onto a second DEAE-cellulose column (0.5 × 4 cm) pre-equilibrated with 15 mM Tricine-Tris (pH 8.0) and 0.05% DDM, mainly to concentrate it for a second sucrose gradient.
Mr. Motian began to worry for him, and stepped in, asking Mr. Kikuchi if he could concentrate on it for a few weeks.
A cub reporter for a West Haversack newspaper discovered Alben's most unusual gift; which is that when given an ordinary house key, Alben, after concentrating on it for awhile can return it to you perfectly straight.
It's a dense work of analysis, lacking the propulsion we associate with the narrative histories of Antony Beevor or John Keegan, so even when immersed in the book — after a purchase-to-start-it lag of several months — I was unable to concentrate on it for more than an hour at a time.
You will be able to learn the lines easier if you are enjoying it, otherwise, you will be bored and not able to concentrate on it for long.
In Alabama, the Legislative Black Caucus filed a lawsuit alleging the Legislature made minority districts too concentrated, making it harder for African-Americans to vote alongside like-minded white voters and diluting their power in the process, according to the Montgomery Advertiser.
"There is definitely the potential for unrest, particularly as the people most affected are the urban poor and they're concentrated, so it's easier for them to organize than it would be for farmers, for example, to organize to protest lower prices," said Nicholas W. Minot, a senior research fellow at the International Food Policy Research Institute in Washington.
They will have to offer a working environment that attracts younger people (such as IBM's design studios in London and elsewhere), develop new products together with their customers and become nimbler to keep up with more focused startups, says Ralf Dreischmeier of the Boston Consulting Group.IBM had a late start mainly because it concentrated for far too long on its financial goals.
