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The fluorescence was compared to insects that ingested inactivated 2 fold concentrated human sera.
The fluorescent dye propidium iodide was used to access the death of epithelial cells exposed to the complement from the 2 fold concentrated human sera.
The fluorescent dye propidium iodide was used to access for the death of epithelial cells when exposed to the complement present in the 2 fold concentrated human sera.
Starving fourth instar nymphs were forced to ingest 50 µL of 2 fold concentrated human sera containing 2.5 µg/ml of propidium iodide.
When the insects were forced to ingest two fold concentrated human sera, the inhibitors inside the midgut were not sufficient to prevent the intestinal epithelium from the complement attack.
Figure 1-G shows groups of dead cells marked in red by the propidium iodide dye and, Figure 1-H shows the absence of marked areas in insects that ingested inactivated 2 fold concentrated human sera.
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