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Sucrose gradient fractions were concentrated fourfold and buffer exchanged into 25 mM Hepes-NaOH, pH 7.4 using Vivaspin 500 centrifugal concentrators (Vivascience).
To obtain a detectable amount of urinary fH, the urine samples were concentrated fourfold by Ultrafree-MC Centrifugal Filter Units (Millipore, Bedford, MA, USA).
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Samples were immediately mixed with 33% (v/v) fourfold concentrated stop buffer.
How concentrated?
He concentrated.
I concentrated.
"I was very concentrated.
Intensely concentrated.
concentrated position.
It decreased side effects, increasing maximum tolerated dose fivefold, and increased the inhibition of tumor growth by concentrating etoposide at the tumor site (a fourfold higher concentration in tumor than with free etoposide).
Concentrating harder.
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