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To combine the concentration step with the detection step, M13 was first concentrated following the same protocol as mentioned previously in Partitioning and Concentrating M13.
Total RNA was extracted, purified from DNA, and concentrated following Lindell et al. (2005).
Proteins were then concentrated following dialysis to >5 mg/ml (>100 μM).
A 0.35 g tissue sample from each case was ribolysed, purified, Proteinase K treated and PrPSc concentrated following the kit instructions and reagents supplied.
Tissue (0.35 g) from each sample was ribolysed, purified, PK treated and PrPres concentrated following the kit instructions and reagents supplied.
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Our studies using concentrates following enzymatic hydrolysates from three common bean varieties of P. vulgaris L., plus black (PB), azufrado higuera (AH), and pinto saltillo (PS), show evidence of antimicrobial activity.
Now, though, their attention is concentrated on following up their Olympics success.
We need to arrive there, respect our supporters and respect ourselves – fully concentrated and following our plan to try and hurt them".
This means precious resources can be concentrated on following these individuals, with prompt treatment of hypertension, urinary tract infections, kidney stones and other complications [ 11]; and not those unaffected.
A simulated gastric solution was made containing 0.4 M glycine (free base, reagent grade glycine in deionized water), adjusted to pH 1.5 ± 0.05 using HCl (trace metal grade concentrated HCl) following the procedure developed for IVBA studies of Pb in soil (29).
A Sep-Pak®Plus C18 column (Waters) purification was carried out to desalt and concentrate peptides following the manufacturer's instructions, followed by solvent evaporation to dryness.
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