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These fractions were combined and the protein solution concentrated (final volume of ~0.5 ml) by centrifugation through a pre-rinsed Vivaspin 6 centrifugal concentrator (5 K molecular weight cut off; Sartorious AG, Bohemia, NY).
Fractions that contained HTkA or HTkB, identified by Commassie-blue staining after electrophoresis of aliquots through 22% denaturing polyacrylamide gels, were pooled and concentrated (final volume of ~2 ml) by centrifugation again through pre-rinsed Vivaspin 6 centrifugal concentrators (5 K molecular weight cut off).
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The concentrated dose of fibrinogen provided by fibrinogen concentrate (final concentration 10 times higher than FFP) might be preferable to FFP for restoring plasma fibrinogen levels because of its rapid availability (no thawing), reduced volume (faster infusion time) and increased safety, and it has recently been suggested to be more effective than administration of FFP [ 15].
Unlabeled protein samples for X-ray absorption near edge spectroscopy (XANES) were concentrated to final concentrations of 0.3 0.7 mM.
The monomeric form was concentrated to final stock concentration by using Vivaspin Ultrafiltration devices (Sartorious, Epsom, UK).
UCONN'S HOLD ON NO. 1 Remains Firm: Connecticut remained No. 1 in the Associated Press poll by a wide margin, following a quiet week in which many players concentrated on final exams instead of on games.
Spore suspension was concentrated to final 1 mL volume.
We could demonstrate that plastic contaminants are released into the sample not only from the depletion column itself, but also when filtering the plasma at the preliminary step and when concentrating the final depleted sample with the provided concentrator (see methods section).
Finally, samples were concentrated to a final volume of 1 mL with a Vivaspin6, centrifugal concentrator, as described above.
Pelleted material was extracted in 1/10 original volume SDS-PAGE buffer to concentrate the final IP fraction.
Fractions containing CaRF as determined by Western analysis were pooled and concentrated to a final concentration of 100ng/uL.
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