After elution, antibody eluates were filtered (0.22 µm), concentrated and exchanged into RPMI 1640 using pre-sterilized AmicronUltra-15 tubes (30-kDa cutoff; Millipore, Ireland).
After gel filtration, protein was concentrated and nucleotide exchange (GDP or GTPγS) was performed as described above for lipidated Gαi1.
The final concentrated volume was exchanged with at least 1.0 L of 20 mM Bis-Tris pH 6.5 to remove residual peptides and other low molecular weight debris.
GST fractions were buffer exchanged to low salt concentration and concentrated with Amicon Ultra-4 centrifugational filters (5K NMWL membrane) and protein concentrations of Amicon concentrates were determined using the NanoOrange protein quantitation kit.
And when you're playing opposite the most self-contained, intuitive actor this country has yet produced (Rylance), you have to boil all emotion, intent and truth down to a single concentrated droplet in every exchange.
The isolation procedure involved tissue homogenization, followed by pressure dialysis, gel filtration of concentrated pressure dialysates, cation exchange chromatography, and thin layer chromatography.
By exchanging the concentrated ammonium hydroxide base solution at 72 h with the dilute solution, constant nitrogen restriction was attained through most of the production period (Figure 7a).
The dichloromethane (DCM) method was similar to the H A method, except the SRS mixture had two components rather than 14, the extraction was performed with DCM rather than H A, the concentrated extract was solvent exchanged into ethyl acetate, there was no SPE cleanup step, and there was only one IS (p,p = -dibromophenyl).
In RED, salt ions are transported from the concentrated salt solution through ion exchange membranes toward the less concentrated solution to extract the energy.
An effective strengthened-glass fabrication method was developed, which involves combined reinforcement incorporating ion exchange using dispersed concentrated potassium nitrate powder, followed by quenching at 15 K/s.
