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The pKM101 CC eluted from the StrepTrap HP column (GE Healthcare) was concentrated down to 500 μl at a final concentration between 5 and 10 mg/ml depending on the preparation.
Subsequently each 2 ml protein fraction was dialysed against de-ionised water using Spectra/Por 3 dialysis tubing with a MWCO of 3.5 kDa (Spectrum Laboratories, Inc., Rancho Dominguez, CA, USA) and then concentrated down to 0.5 ml using a vacuum concentrator (Speed-vac SPD131DDA, Thermo Scientific, Basingstoke, UK).
This summer, Bt corn covered an estimated 22 million acres concentrated down the center of the Corn Belt in the Dakotas, Nebraska, Minnesota, Iowa, Kansas and Oklahoma.
Eluate was buffer exchanged into 1× PBS/20% glycerol and concentrated down to 150 µl volume.
The NA-containing fractions were pooled and concentrated down for further analysis.
The plush toys were then extracted in 200 mL of hexane via sonication and concentrated down to a sample volume of 5 mL (Gurunathan et al. 1998).
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Short-period seismic wave back-projections indicate the source region that radiated short-period teleseismic signals is concentrated down-dip from the hypocenter, near the coastline (e.g., Koper et al., 2011).
Resulting elutant was then dialyzed again in a solution of 0.1X PBS/9 mM HEPES pH 7.4 containing 0.05% Tween-20 (Fisher) before concentrating down to a desired level.
So we're looking for ways to concentrate down the sample over time.
"He can concentrate down here," Rothschild said, pointing to the lower part of the strike zone.
But there's no doubt that the national public media is being concentrated, slimmed down and is now managed by government appointees.
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