As a result this fluid in the medullary and deep cortical regions of the kidney becomes highly concentrated, reaching concentrations of up to four times that of the plasma (1,200 mosmoles per litre), mainly owing to the accumulation of sodium and chloride.
Both Ku and TKu were concentrated and concentrations determined using the Micro BCA Protein Assay Kit (Pierce) using BSA as standard and further confirmed by UV absorbance.
The constructs were eluted with a 0 500 mM gradient of imidazole concentrations, concentrated using a 5 kDa-cutoff concentrator (Millipore) and dialyzed against 10 mM Tris-HCl, pH 8.0, containing 200 mM NaCl.
The objectives of this study were to determine the effects of dietary forage source with two concentrate concentrations on dry matter (DM) intake, rumen fill, ruminal and intestinal digestibility of nutrients, and duodenal N fractions in lactating cows.
Here he concentrates on concentration itself.
Unlabeled protein samples for X-ray absorption near edge spectroscopy (XANES) were concentrated to final concentrations of 0.3 0.7 mM.
These were then concentrated to high concentrations by ultrafiltration to verify the validity of the criterion.
The J591c-scFv was purified from HEK293T culture supernatant by Ni-NTA chromatography (5 ml Ni-NTA Superflow Cartridge, Qiagen, Manchester, UK); the collected fractions were concentrated by centrifugal concentrations, then further purified by FPLC gel filtration using an AKTA system (Superdex 75 HR 10/30, GE Healthcare, Little Chalfont, UK).
Combined protein-containing fractions identified by SDS PAGE were concentrated using centrifugal concentrations (Amicon, 10 kDa MWCO) and applied to a Hi-Load 26/60 Superdex 75 size-exclusion column using an Akta Purifier FPLC system.
Between 10 and 25 μl (depending on the DNA concentration) were concentrated in a Speed Vac (Thermo Scientific) to increase the DNA concentration to at least 1 ng/μl.
