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After addition of 8 μl of a 5-fold concentrated compound solution (DEX, FA, or TA) in assay medium, the cells were incubated for additional 5 hr.
Dose response experiments were performed in the same way, except that concentrated compound stocks were used (10 μM in DMSO) that were serially diluted in 100% DMSO in half log series before addition to the assay plate.
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Along these same lines, it was also possible that the concentrated compounds in the CR concentrate could be buffering pH of the media and mitigating the negative lifespan effects of acid accumulation by WT yeast grown under NR conditions.
Concentrated compounds (10X) were injected into each well using the internal injector of the cartridge and three successive 2 min measurements were performed at 3 min intervals with inter-measurement mixing.
Although the overall sensitivity with the small volume of mouse serum prevents the detection of large numbers of metabolites, we were able to obtain high-quality NMR data on the most concentrated compounds, suggesting that C NMR metabolomics using isotopically labeled compounds for flux studies on survival bleeds is also possible with this probe.
Data consisting of the relative proportion of all compounds were transformed (log (x-1)) to reduce the large difference of abundance between highly and slightly concentrated compounds and standardized (mean = 0, standard deviation = 1) to reduce the sample concentration effect [ 82].
Healthy nonsmokers typically had acetone (ATM-C3H6O in Fig. 2c) as the most concentrated carbonyl compound in their exhaled breath.
Chlorogenic acid is the most concentrated polyphenolic compound in the fruit cortex of the apples from the 'Royal Gala' × 'Braeburn' segregating population.
Treatment of this water usually requires using high concentrated chlorine compounds, which may cause a high risk of human cancer [6].
The OCPs and PCBs were the most highly concentrated POP compounds, whereas chlordanes and HCHs were generally found at trace levels.
While essential oils are natural solutions, they are still highly concentrated chemical compounds.
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