Sentence examples for concentrated cells from inspiring English sources

In addition, a novel set of 75 nanoLC-MS/MS runs were acquired from samples prepared from five cellular extracts of cells grown in exponential phase in marine broth and then subjected to UV stress, concentration (100× concentrated cells), or grown on benzoate or in presence of naphthalene, and then resolved by SDS-PAGE.

Cyt- c-released cells (cyt- c was diffusely expressed in cytosol) and cyt- c concentrated cells (cyt- c was concentrated relatively in mitochondria around the nucleus) were respectively counted in 10 randomly chosen fields.

Concanavalin A-FITC (Sigma-Aldrich, St Louis, MO) was added to the concentrated cells to a final concentration of 0.2 mg/ml.

The cell suspensions collected were then concentrated through centrifugation at 500 × g for 10 min. Aliquots of the concentrated cells were then used for cell counting by a hemocytometer, flow cytometry analysis, as well as for further studies.

After the process, DNA was extracted from the concentrated cells, and PCR was applied to detect E. ictaluri.

Cell number was determined using the a Thoma counting chamber (Paul Marienfeld GmbH & Co. KG, Lauda-Königshofen, Germany) and adjusted to 2 × 106 cells ml−1 by the addition of concentrated cells to the cell-free supernatant.

Immediately prior to the labeling reaction, MgCl2, MnCl2, and CaCl2 were added to the concentrated cell lysate until their final concentrations reached 50, 5, and 5 mM, respectively.

Take 0.1 ml of the concentrated cell suspension and dilute it to an approximate concentration of 1-2 × 10 cells/mL.

However, since cell growth is also significantly modulated by the nutrient contents, intense cell motility with conventional uniform cell seeding method may lead to declined cell growth in the final time because concentrated cell population has been growing around the scaffold periphery to block the nutrient transport from outside culture media.

The 4 mL concentrated cell solution was centrifuged for 15 min at maximum speed.

Total cell lysates and pre-filtered, 10-fold concentrated cell supernanants were subsequently processed for Western Blot analysis as previously described [20].