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It is possible that we artificially increased the probability of loading the highly concentrated cargo within the carriers.
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The multiple Atg8 binding sites in Atg19 subsequently mediate the selective interaction with membrane localized, locally concentrated Atg8, enabling Atg19 to bend the membrane tightly around the cargo and to exclude non-cargo material from its delivery into the vacuole (Baba et al., 1997; Sawa-Makarska et al., 2014).
CME is a concentrative uptake mechanism so that B-cargo and Tfn will be ∼20-fold more concentrated in nascent CCV derived vesicles as compared to bulk PM proteins in CLIC.
NDRG1 strongly interacted with phosphatidylinositol 4-phosphate (Figure 5C), a lipid concentrated and maintained by ARF1 in the Trans Golgi Network, and is involved with vesicle formation, transportation and sorting cargo proteins to endosomes [30].
The cargo of EVs isolated from tumor cell culture media can be bioactive because injection of concentrated tumor-cell-line-derived EVs educates tumor-supporting cells, such as bone-marrow-derived cells, that subsequently prepare sentinel lymph nodes and lungs for metastasis (Hood et al., 2011; Peinado et al., 2012).
The function of these binding sites seems to be to produce a concentrated cloud of the factors around the entrances of the NPC to make the various processes of cargo loading and release more efficient (reviewed in Strambio-de-Castillia et al. 2010; Wente and Rout 2010).
More concentrated.
Cargo pants!
That concentrated minds wonderfully.
They are extremely concentrated.
"So focused, so concentrated".
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