Sentence examples for concentrated by using from inspiring English sources

Purified Rfa2 was concentrated by using centrifugal concentration tubes (Amicon, Millipore) until a final concentration of 0.8 1.0 mg/ml was reached.

Heme precipitate was removed by centrifugation, and the protein was concentrated by using an Amicon centrifugal concentrator (Millipore) to a final volume of 90 100 mL.

All stool specimens were concentrated by using the formalin-ethyl acetate concentration procedure routinely used to examine ova and parasites in stool specimens (2 ).

Appropriate fractions were pooled, dialyzed against water and concentrated by using Vivaspin Turbo 15 (cut-off 10 kDa) centrifugal concentrators (Sartorius, Göttingen, Germany).

Volatiles were extracted and concentrated by using preconditioned SPME fiber at 250 °C for 30 min attached to the SPME manual holder (57330-U) (Supelco, Bellefonte, PA, USA).

The supernatant of liquid cultures of E. coli clones expressing wild-type rCelStrep and the selected mutants was concentrated by using the stirred ultrafiltration Amicon® system (Millipore Corporation, Bedford, MA, USA) with a 3 kDa polyethersulfone membrane.

The dialyzed sample was concentrated by use of Centripreps (Millipore) to a final concentration of 26 mg mL−1.

Total RNA was isolated from roots with use of an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), treated with DNase (Roche, Germany) to eliminate genomic DNA contamination, then purified and concentrated by use of the RNeasy MinElute Cleanup Kit (Qiagen).

Supernatant was harvested from transfected 293FT cells 24 hours following transfection, filtered using a 0.22 µm sterile filter, and concentrated by centrifugation using a Beckman SW32 rotor at 30,000 rpm.

The NarP protein was concentrated by ultrafiltration using an Amicon Ultra-15 column and the protein concentration was determined using a Bradford assay [54].

Peak fractions were concentrated by centrifugation using a 30 kDa cutoff membrane concentrator unit to 5 10 mg/mL.