Sentence examples for concentrated by three from inspiring English sources

The reaction was stopped by adding glycine to reach a final concentration of 10  μM; fluorescent hPrP90-231 (hPrP90-231-FITC) was separated by unbound FITC and concentrated by three centrifugation steps in Millipore (Cork, Ireland) centricon tubes (10 ml, 10 kDa mesh).

The solar radiation is both collected and concentrated by four Fresnel lenses, and redirected towards a laser head by four plane folding mirrors.

Virus-containing supernatant was harvested 48 h after transfection and concentrated by two rounds of ultracentrifugation.

Virus-containing supernatant was harvested 48 hr after transfection and concentrated by two rounds of ultracentrifugation.

The chitinase produced by the R. stolonifer is extracellular chitinase which was secreted into the medium, so it can be concentrated by the two methods i.e. fractional ammonium sulphate precipitation and organic solvent precipitation.

After cooling and filtration, the extract was frozen and concentrated by lyophilization for five days overnight, in order to obtain the ABE (0.68 g).

Conversely, proteins involved in protein folding, degradation and stress responses were concentrated by a factor of three (Additional file 2: Table S2).

Afterwards, the crude extract was concentrated by ultrafiltration and separated by five chromatographic steps using an FPLC system and strong anion exchangers (Q Sepharose FF, Mono Q 10/100, Source 15Q, Mono Q 5/50 columns) as well as a molecular sieve (SEC Superdex column) (Table 1).

The adenovirus preparations then were purified and concentrated by CsCl2 gradient ultracentrifugation two times.

Samples were concentrated by centrifugation through Microcon YM-10 filters (Millipore).

Cells grown to exponential growth phase in YNB medium was concentrated by a factor of ten by centrifugation.