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Molecules less than 5 kDa in molecular weight were not concentrated by this method; nevertheless, the fluid still contained these substances in concentrations found before ultrafiltration and the concentrated BALF represents alveolar epithelial fluid better than unprocessed BALF.
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Then, the mixture was washed and concentrated by the method as mentioned above.
The VLPs purified and concentrated by the method schematically presented in Fig. 1a, panel B, were of the best quality and were subjected to further analysis.
However, Pd diffuser is suitable for the secondary process, like the purification process, after the tritium in the sweep gas has been concentrated by another method.
Cider samples were concentrated by centrifugation, and water samples were concentrated by Environmental Protection Agency method 1623 (8 ).
These water samples were concentrated by a method slightly modified from Gilgen et al. (22 ).
For biosurfactant extraction, the cell-free broth was concentrated by a precipitation method (Youssef et al. 2007a, b).
The source of the DOM is from fresh water in Northern Europe, and the final DOM product has been concentrated by a proprietary method and is commercially available from FBSciences, Inc.
RNA was concentrated by the precipitation method of isopropyl alcohol, purified by the NucleoSpin RNA clean-up kit.
The remaining PAH and oxidation products were extracted from the filters, pre-separated (using solid-phase extraction) and concentrated by applying a method developed earlier [ 27, 28].
The resultant production of lentivirus was concentrated by an ultracentrifugation method (20000 ×g for 2 h at 4°C), resuspended in 1% BSA, frozen in aliquots at −80°C, and titered using HEK-293T cells as described previously [ 39] (http://tronolab.epfl.ch/).
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