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The final eluate was concentrated by binding to 10 μl of hydrophobic StrataClean resin (Stratagene) then released into Laemmli SDS-PAGE loading buffer.
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The captured fractions were washed several times with lectin binding solution and concentrated by centrifugation.
The viral supernatants were filtered using a 0.45- μm low-protein-binding filter flask (Millipore, Vimodrone, Italy), and viral particles were concentrated by ultracentrifugation as described (Follenzi et al, 2000).
Viral supernatants were concentrated by PEG precipitation.
First, the mineral is crushed and concentrated by gravity separation.
Briefly, 450 µl of lysate were concentrated by ethanol precipitation.
The protein was concentrated by ultrafiltration and stored at −70°C.
The eluate was purified and concentrated by ethanol precipitation.
The protein solution was then concentrated by ultrafiltration.
Cells were cooled rapidly to 0°C and concentrated by centrifugation.
The microscopic residues were then concentrated by centrifugation.
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