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For sewage water, 100 mL were concentrated by a Molecular Weight Cutoff (MWCO) 100,000 Da Vivaspin20 centrifugal concentrator (Sartorius, Madrid, Spain) to a 5-mL concentrated sample.
This eluted solution was concentrated by a stream of nitrogen to 1 mL. 1 μL of the concentrated solution was spiked with 1 μL of 100 ppm internal standard exactly before an injection to the GC-ECD.
After the purification of the barium-radium chloride, radium is concentrated by a process of fractional crystallisation, radium chloride, less soluble than barium chloride, being concentrated in the crystals.
The X-rays were monochromated to 2.1655 ± 0.0009 Å and were concentrated by a short-gap undulator.
For biosurfactant extraction, the cell-free broth was concentrated by a precipitation method (Youssef et al. 2007a, b).
The possibility of baking on a glass stove is investigated using solar thermal energy transferred to the kitchen by means of a circulating heat transfer fluid heated by solar energy concentrated by a parabolic trough.
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The prime minister's mind has, of course, also been concentrated by an imminent election, and that it is the first since 1997 that Labour is at serious risk of not winning.
The collected fractions were subjected to buffer change by ice-cold Homogenization Buffer without sucrose and concentrated by an Amicon Ultra centrifugal filter device (Millipore).
Vaccine antigens produced from the 7.5 L bioreactor were harvested through a 0.65 µm depth filter; and the clarified solution was then concentrated by an ultrafiltration step using 300K membrane (Sartorius, Germany).
The recombinant MfHEs were purified by Ni-NTA Superflow chromatography (Qiagen, Valencia, CA, USA) or concentrated by an Amicon Ultra-15 (Millipore Co., Billerica, MA).
Corneocytes adherent to the tape surface were eluted by 5 ml of urea-buffer and concentrated by an Amicon-ultra 10 kDa (Millipore) into 50 µl.
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