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Crude culture liquids were concentrated approximately tenfold by volume.
In PRP preparations, platelets were concentrated approximately 6.8-fold over PPP preparations.
Viral supernatant was concentrated approximately 100-fold by centrifugation at 6000 rpm for 20 min at 4°C.
The medium was then concentrated, approximately 25 fold, using ultrafiltration units (Amicon Ultra-PL 3, Millipore, Bedford, MA, USA) with a 3 kDa molecular weight cut-off.
This combined medium was then concentrated approximately 200-fold through a centrifugal filter device with a 3 kDa molecular weight cutoff.
Equal volumes of conditioned medium from BACE1 overexpressing and control cells were combined and concentrated approximately 200-fold using Centricon filter devices with a 3 kDa cutoff (Millipore).
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Metagenomes were prepared by concentrating approximately 60 l of seawater using a tangential flow filter (tff).
The Genomic DNA Clean & Concentrator Kit Zymo Research Corporationn, Irvine, California, USA) was used to concentrate approximately 600 μL of gDNA into a 30-μL volume.
At present, the catch of gangfisch for production of fry is concentrated around depths of approximately 20 m (information from local fishermen).
P14 soluble TCR eluted at approximately 15.5mL and was concentrated to approximately 5mg/mL by centrifugal concentration (Amicon centricon).
Soluble P14 TCR eluted first at approximately 150mM NaCl and was concentrated to approximately 1mL by centrifugal concentration (Amicon centriprep) prior to size-exclusion chromatography on a Superdex 200 HR column (Amersham) equilibrated in 10mM Hepes pH7.4, 150mM NaCl, 3mM EDTA, 0.01% P-20.
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