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OECM components with their sizes larger than 30, 50 and 100 kD were concentrated and collected respectively using Centricon Ym-30, 50 and 100 (Millipore, Billerica, MA).
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The details of the new designing method and homemade unified code with MCRT for numerical investigations on solar concentrating and collecting characteristics of the CSCs are introduced.
A new designing method and homemade unified code of the Monte Carlo Ray-Trace (MCRT) Method for numerical investigations on solar concentrating and collecting characteristics of the PVR is proposed.
The described setup is capable of rapidly concentrating and collecting nanoparticles from continuous flow that is driven by electroosmosis.
The enzyme was collected, concentrated and partially purified from the culture supernatant.
The fraction eluting at Rt = 10.5 min was collected, concentrated, and resuspended in PBS/ethanol (95/5) for all radiotracer bioassays.
Eighty (50 mL) fractions were collected, concentrated and grouped based on TLC profile.
Sixty (50 mL) fractions were collected, concentrated and grouped based on TLC profile.
The enzyme fractions were collected, concentrated, and exchanged with 10 mM phosphate buffer (pH 7.0).
The glucose octanoate respectively xylose octanoate fractions were collected, concentrated, and transferred to pre-weighed round-bottom flasks.
The refolded proteins were further purified by gel filtration chromatography at 4°C and the peaks with >95% purity were collected, concentrated and stored at −80°C.
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