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The concentrated active fractions were stored in 100 mM phosphate buffer (pH 7.0) containing 10% glycerol at −20°C.
SDS-PAGE analysis of a concentrated active fraction revealed four major protein fragments (data not shown).
Dried leaves of Lawsonia inermis were used to prepare the extracts as it has been reported that dried preparation have more concentrated active phytochemical compounds than fresh plant material [ 25].
As shown in Figure 1, SDS-PAGE of the partially purified bacteriocin (concentrated active FPLC-fraction) showed a diffuse band on the Coomassie-stained gel while a clear inhibition halo was visible on the activity-gel for the concentrated FPLC-fraction as well as for the CFS.
They contain highly concentrated active agents and are a must when it comes to skin care.
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These data indicate that the Endopep-MS method is very sensitive with respect to the amount and concentration of toxin and that methods that can concentrate active toxins into smaller reaction volumes (thus yielding higher concentration) will result in lower limits of detection.
Moreover, the majority of propagating optical modes is concentrated around active material region that results in compact, enhanced modulator design.
Most of the remainder is also concentrated among active criminals; ordinary citizens who own guns do not commit street robberies or shoot their neighbors and wives.
However, qualitative observations of xanthophore morphology were made using methylene blue, which is taken up specifically by xanthophores and is concentrated around active pterinosomes, the organelles that produce pteridine pigments [60].
With this apparatus, 50 µL of normal sera or 2 fold concentrated sera (active or inactivated by 30 min at 56°C) were injected into the digestive tract of the bug.
We show that oxidized DNA and lipids are concentrated within active portions of the lesions.
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