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Briefly, protein spots were digested using 150 ng of trypsin, peptide extraction was performed using 5 sonication cycles of 2 min each and peptides were concentrated 1 hour at 50°C in a heat block.
Approximately 1 year after passive sampling, we collected and concentrated 1 L of water from the same locations.
The total 70% ethanolic extracts were concentrated (1 L) and successively partitioned with ethyl acetate (1 L × 3 times) and n-butanol (1 L × 3 times) to yield three sub-fractions, designated as the EA, BuOH and Water fractions.
The monoclonal anti-EGFR-antibody Cetuximab (Erbitux®, ImClone Systems, Bristol-Myers SqUSAb, New York, und und Merck KGaA, Darmstadt, Germany) concentrated 1 μg/ml was directly added in experiments evaluating the ADCC of NK cells.
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For MALDI-TOF analysis of the active HPLC fractions 20 µl of each fraction were concentrated 1∶10 using a rotational Vacuum Concentrator (RVC 2 18, Christ, Osterode, Germany).
Cage water was concentrated 50-fold in a vacuum concentrator.
Media were concentrated (~20X concentrated) using a Centricon concentrator (Amicon, Beverly, MA, usa) and quantitated as described previously [ 34].
We further concentrated DGAT1 with ultra-filtration (Fig. 2A).
To satisfy the need of highly concentrated 188Re-perrhenate for the clinical applications, we designed an apparatus to achieve the purpose of concentrating 188Re-perrhenate solution.
KMnO4, NaNO2, NaOH, concentrated H2SO4, concentrated HCl, and H2O2 (30%) were all analytical grade and purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
Study of antifungal activity of adjusted to pH 6.5 supernatant concentrated (10×) by evaporation and concentrated (22.5×) by lyophilization did not show any positive results.
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