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The concentrate was applied to Sephadex G-150 column (70 cm × 1.8 cm) pre-equilibrated with 10 mM acetate buffer (pH 4.8) and the fraction were collected at a flow rate of 0.4 ml min−1.
Over the 24-hour period, a cumulative median dose of 7 g fibrinogen concentrate was applied.
The protein concentrate was applied to a HiLoad 16/60 Superdex 200 PG column connected to the AKTAxpress chromatography system (GE Healthcare) for further purification of Xbra-His10 through gel filtration in 25 mM sodium phosphate pH 8.0 and 150 mM NaCl.
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Before staining, IMS concentrates were applied to Dynal Spot-On slides (Dynal Biotech, Oslo, Norway) and air dried.
The concentrating approach was applied on Cu In,Ga Se2 to develop photovoltaic devices with increased efficiency using less rare materials.
The purified and concentrated antimicrobial peptide was applied onto the gel in duplicate.
A TM010 microwave resonance cavity was applied to concentrate the microwave energy.
The Amicon Ultra-15 column (Millipore) was applied to concentrate the proteins in a PBS buffer, pH 7.5 using a modified manufacturer's protocol.
In the present work, continuous-effect membrane distillation (CEMD) process with a function of in-situ internal latent-heat-recovery was applied to concentrate aqueous glycerol solution by using a hollow fiber air-gap membrane distillation module.
If necessary, ethanol precipitation was applied to concentrate the DNA sample.
To optimize the thermal performance of the modular MFPA, a nozzle was applied for concentrating airflow.
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