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In the former cases, 2 ml of MSCs concentrate was added to 1 g of collagen powder and 1 ml of platelet-rich fibrin gel (obtained the day before surgery).
24h after medium containing viral concentrate was added, it was replaced by fresh complete neurobasal medium, without viral concentrate.
Briefly, 40 μL of Coomassie Plus reagent concentrate was added to 160 μL of dispersion of nanoparticles, either mAb-coated or noncoated.
Briefly, 50 µL of viral concentrate was added to a mixture of 50 µL of silica particle suspension and 900 µL of lysis buffer.
After 3 washes with washing buffer, 100 μL of diluted HRP-conjugated streptavidin concentrate was added to each well and the plate incubated at room temperature for 30 min.
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Haemostatic agent (therapeutic plasma/cryoprecipitate/fibrinogen concentrate) is added 1 unit at a time until the target fibrinogen level or the specified amount of haemostatic agent is reached.
Decreased coagulation rate and storage modulus (G′) were observed when denatured whey protein concentrate (DWPC) was added to milk.
For each sample 3 ml of concentrate HNO3 was added and digested in an electrically heated block for 1 h at 145 °C.
Concentrated buffer was added to restore the buffer concentrations to 20 mM Tris-HCl, pH 8.0, 1 mM DTT, and 1 mM EDTA.
Concentrated NaCl was added to the lysate at a final concentration of 0.5 M. The solution was loaded onto a Ni-affinity column (Ni Sepharose 6 Fast Flow; Amersham) equilibrated with Column Wash Buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl).
About 1 2 mL concentrated HCl was added drop wise over 5 10 min in a stirred mixture of ketone.
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