Upon arrival of the self-sampled material in the laboratory, tubes were centrifuged to concentrate the cell material from the lavage specimens.
To concentrate the secretory material, the media samples were centrifuged through Vivaspin columns (Sartorius Stedim Biotech) that trap material above a certain molecular weight.
The pellets were treated with RNase (Ambion, 20 µg/mL) and DNase (Invitrogen, 10 U/µL) (both from Life Technologies, Grand Island, NY, USA) to concentrate the viral material.
Although different types of natural hazards inflict varying levels of damage on the different elements in an exposed area, these physical structures, activities, and resources concentrate the majority of the material wealth and thus are the main objects of damage assessment.
'When we discussed getting back together, we agreed that we would concentrate on the material without any of the old clashing of egos,' the likeable Steve Jansen told me when we met for coffee.
For reactions of negative order, such as MGP oxidation, at high bulk oxygen concentrations (CO2,L>0.3 mol/m3), concentrating the active catalytic material in a layer buried some distance from the surface (core) gives considerable better performance than the conventional "egg shell" design of shallow deposition near the surface or uniform distribution.
The exhibition concentrates on the material culture of the Ming aristocracy; regrettably few artefacts from the bottom rungs of the social ladder survive.
The procedure eliminated nearly all distraction and concentrated only the material I had to deal with in a given day or week.
Early implementation of MPM assumes that the mass is concentrated at the material points, which causes noise in the solution.
The band spent the first half of 2005 concentrating on the material for the new album Remagine, while Borgman received intensive treatment to cure his cancer.
