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The animals' diet was supplemented with 150 g of standardized concentrate composed of 770 g/kg barley, 200 g/kg wheat bran, and 30 g/kg commercial mixed minerals and vitamins.
The diet ingredients used were: Tifton 85 hay (roughage source), and a concentrate composed of ground corn, soybean meal and urea, together with 100 g/kg of dry matter (DM) of crude glycerine (CG) or 60 g/kg DM of soybean oil (SO) or 100 g/kg DM crude glycerine plus 60 g/kg (DM) soybean oil (CGSO).
Five ruminally cannulated Nellore steers were randomly assigned in a 5×5 Latin square design and fed diets containing 30% corn silage and 70% concentrate composed of sunflower meal, corn grain, soybean hulls, minerals, and 0, 7.5, 15, 22.5, or 30% crude glycerin (860 g glycerol/kg).
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Pelleted concentrate was composed of ground corn, soybean meal, a mineral vitamin supplement for goats, salt and CSFA at the levels of inclusion.
The concentrate was composed of ground corn (46.7% DM basis), soybean meal (10% DM basis), urea (1.24% DM basis), limestone (0.50% DM basis), salt (0.25% DM basis) and a commercial mineral supplement (1.3% DM, Minerthal MD ®).
The concentrate, primarily composed of media constituents with molecular cut-off of 100 kDa or 10 kDa comprises the cell culture supernatant derived protein rich extracellular matrix.
Cows were individually fed a corn silage-concentrate based diet composed of 93% roughage and 7% concentrate (DM basis) as a total mixed ration.
One group was fed with a high-concentrate diet (HC) composed of 40% forage and 60% concentrate as a treatment, and the other group was offered a low-concentrate diet (LC) composed of 60% forage and 40% concentrate as a control for the 18-week experimental period.
Briefly, a group of 31 cows were fed a mixed diet composed of concentrates + alfalfa and corn silages with the addition of 3.6% corn oil.
The experimental diets were composed of 50% concentrate and 50% forage.
Drops were a watery mixture composed of cholecalciferol concentrate in oil, citric acid monohydrate, star anise oil, potassium sorbates, polysorbatum 80 (polyoxyethylene sorbitan monooleat), sugar syrup, and purified water.
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