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The O/W emulsion was formulated from a commercial oil concentrate (1 wt % in distilled water) used in metalworking processes.
Half of the cows were supplemented with a mineral/vitamin mixture (1 kg/ d of the mix in a corn/molasses carrier) and the other half supplemented with a corn-based concentrate (1 kg of concentrate per 4 kg of milk).
The fermentation medium consisted of (g L−1): glucose 60, yeast extract 10, glutamic acid 1, MgSO4 7H2O 0.1, KH2PO4 0.5, CaCO3 0.05, and vitamin concentrate 1 mL (0.5 mg vitamin B12, 0.5 mg vitamin H, 1 mg vitamin B1, dissolved in 1 L of water).
Twenty crossed (Bos taurus×Bos indicus) steers (270±30 kg BW) were used in a grazing trial (80 days), feeding individually concentrate (1 kg/d, 14% CP) and urea treated sugar cane (USC), in a completely randomized design according to the following treatments: (1) Grazing control (GC); (2) USC-0; (3) USC+15 g d−1 Fibrozyme (USC-15); (4) USC+30 g d−1 Fibrozyme (USC-30).
*Platelet (4 units) and Red cell concentrate (1 unit) were transfused on 14/7/2011.
Figure 2 shows SEM observation of GRHC pellets seeded with the BM aspirate or the BM concentrate 1 day after plating.
Similar(52)
Subsequently, a detector antibody (rabbit anti- B. globigii), recognizing another epitope on the B.g. spore surface, was diluted in BSA diluent/blocking solution concentrate (1 15 dilution in distilled water) to a final concentration of 5 μg mL−1 and incubated at 37 °C with the captured spores before being washed several times (PBS + 0.5% Tween 20).
Retentate concentrate 32.
Milk concentrate 26.
The following experimental diets were used: (1) 60% corn silage+40% concentrate, (2) 40% corn silage+60% concentrate, (3) 60% fresh sugarcane+40% concentrate, and (4) 40% fresh sugarcane+60% concentrate.
Skim milk concentrate 37. Skim milk retentate 38.
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