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CON: Protein powders may contain hidden ingredients that could mess with your goals.
A blast search of the Genbank viral sequences database of the HA 1 5 con protein sequence revealed the closest influenza isolates had only 69% identity.
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Alignment of HA amino acid sequences showed that the HA1-con protein conserved functional elements that include cleavage, fusion, transmembrane and cytoplasmic domains (Figure 2).
Phylogenetic analysis shows that the synthetic centralized HA1-con protein localizes to the central region of the H1 tree (Figure 1B).
For each combination we used the following equation: \sum _{m = 1}^{m = {\rm \#\ genes} - 1} \quad\sum _{n = m + 1}^{n = \#\ {\rm genes}} ({\rm Length(}X_m ) - {\rm Length}(X_n ))^2, Cons: The protein isoform combination that results in the best-conserved alignment, measured as percentage of amino acid identity over the length of the alignment (Conservation score).
After calculating all possible combinations, we applied different methods to select a single combination: Cons: The protein isoform combination that results in the best-conserved alignment, measured as percentage of amino acid identity over the length of the alignment (Conservation score).
For our experiments presented in this study, the lambda subtype of AL-LC and kappa subtype of Con-LC proteins were used.
Additionally, under non-reducing native conditions, we see that the majority of AL-LC and Con-LC proteins migrate to a molecular weight consistent with a dimeric state (Supplementary Fig S5B), while under reducing conditions, these proteins are found at a molecular weight consistent with monomeric state (Supplementary Fig S5C).
Using the ROS-sensitive fluorescent dye, DCFDA, we found that increased AL-LC-elicited ROS was abolished with Mito-TEMPO to levels comparable to cells treated with vehicle (Veh) or control light chain (Con-LC) proteins isolated from patients with multiple myeloma (Fig 1A).
At time intervals of 2, 4, 8 and 12 h, [NH3-N] was much lower in CON than in protein supplemental treatments (P < 0.05).
CON: Overloading on protein powders may be harmful.
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