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The axonal growth of RGCs was calculated by a computerized image analyzer.
Histomorphometrical analysis was performed using a camera-equipped light microscope system (Eclipse LV100POL, Nikon, Japan) and a computerized image analyzer (NIS-Elements AR 3.00).
The quantity of DNA/RNA-protein complexes was evaluated by scanning the fluorescent image with a computerized image analyzer, FX Pro (Bio-Rad Laboratories, HerCAles, CA, USA).
Histomorphometric analysis was performed by one experienced investigator blinded to the specific experimental condition, using a Leitz Orthoplan Light Microscope (LM, Leica Microsystem Inc., Bannockburn, IL, USA) equipped with a computerized image analyzer system (Qwin, Leica Microsystem Imaging Solution Ltd ,Cambridge, UK).
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The movements were evaluated by a computerized tracing system and image analyzer (EnthoVision 3.1, Noldus, Wageningen, The Netherlands).
Locomotion has also been studied in mice with CIA using a computerized tracking system and image analyzer (EnthoVision 3.1, Noldus) and was found to be correlated to clinical arthritis [ 18].
Kinematic parameters were evaluated in mouse epididymal extracts to monitor maturation of sperm movement in animals exposed to static magnetic fields using the Sperm-Class Analyzer computerized image analysis system.
Quantification of the bands was performed with a computerized densitometer connected to an image analyzer (Bio-Rad GS700) and is expressed in optical density units per mm.
During the last two decades new computerized image techniques measuring and analyzing ONHs were developed.
Areas were then analyzed with an image analyzer MIP 4 ADVANCED (Digital Image System, Barcelona, Spain).
Images were captured and analyzed using Ziess LSM image analyzer version 4.0.
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