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The diameter of the PHB granules on SEM pictures was evaluated using the ImageJ plugin Fiji (Laboratory for Optical and Computational Instrumentation (LOCI), University of Wisconsin-Madison, WI, USA).
Financial support was also provided by the Morgridge Institute for Research, The Rapid Prototyping Center (RPC) at the Milwaukee School of Engineering (MSOE) and the Laboratory for Optical and Computational Instrumentation (LOCI) at the University of Wisconsin – Madison.
BLC is a graduate student in the Medical Physics Department at UW-Madison, a member of the Laboratory for Optical and Computational Instrumentation (LOCI) at UW-Madison and an engineer in the Medical Engineering Group at the Morgridge Institute of Research.
Images were acquired using WiscScan (http://www.loci.wisc.edu/software/wiscscan), a laser scanning software acquisition package developed at LOCI (Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI) described in previous work [ 15].
The web application was developed using Google Web Toolkit (1.7.0) [ 23], Apache Commons FileUpload package (1.2.1) [ 24], Apache Commons IO library (1.4) [ 25], Laboratory for Optical and Computational Instrumentation Bio-Formats package (4.1) [ 26], and Apache Tomcat servlet container (6.0) [ 27].
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