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Computer-aided and computational image analysis has been performed since computers emerged in the 1940s, with major advances made as the acquisition, storage, analysis and manipulation of images have become cheaper and more accessible.
The term "generalized reference" is used in computational image reconstruction (computer vision).
Recent advances in labeling, imaging, and computational image analysis are allowing quantitative measurements to be made more readily and in much greater detail in a range of organisms (e.g., Arabidopsis, Ciona, Drosophila, C. elegans, mice, Platynereis, and zebrafish) [ 1, 3– 6].
Here, we use in vitro reconstitution assays with single-molecule resolution, live-cell transport assays in primary neurons, computational image analysis, and computer simulations to investigate the factors regulating retrograde transport initiation in the distal axon.
The most recent of these attempts employed a combination of high-resolution imaging (using SEM) and computational image analysis to identify 12 species of extant grass pollen based on the size and shape of sculptural elements on the pollen surface and the complexity of the ornamentation patterns they form (Mander et al., 2013).
OA and AI were calculated by computational image analysis while TB and EE were manually measured.
Similar(37)
Thus, we next pursued negative-stain electron microscopy coupled with computational image-processing methods.
These coats were imaged using an electron microscope, and the images were analysed using computational image-analysis methods.
Computational intensive image enhancement algorithms such as the Retinex [2] and its variants including optimization through variational methods [3, 4] are not discussed.
Note that (ua, va) and (uc, vc) represent the velocity at x and y directions pertaining to the analytical and computational velocity image grid respectively.
Please refer to Additional file 1 for detailed description of computational infrastructure, image analysis, and data normalization.
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