After thawing and washing with IMDM (containing 2% FBS) to remove DMSO, cells were incubated with DNAse to degrade free DNA in solution that would compromise separation efficiency.
The problem of contaminating host tissue has probably been overemphasized: small amounts of contaminating host proteins should not compromise the separation and can subsequently be withdrawn from the dataset when in silico searches show a match with the host organism.
My message is clear: Childhood should not be compromised by parental separation or divorce.
Currently, scientists within the natural history museum have significant input into the design and content of museum exhibits and public programs; they worry that the museum's mission will be compromised by the planned separation.
Opponents stirred up fears among a largely Protestant America that a Catholic in the White House would result in the Pope running the country and compromise the constitutional separation of church and state.
The currently described indications for surgical treatment are open fractures, neurovascular involvement, skin compromise and wide separation of bone fragments with soft tissue interposition.
Movements of 16 to 19 mm of a left double-lumen tube and 8 mm of a right double-lumen tube can compromise functional lung separation [ 33].
However, the flexural strengthening effectiveness of this NSM technique is sometimes compromised by end concrete cover separation (CCS) failure, which is a premature failure before occurring the conventional flexural failure modes.
ApoE ∈4 homozygotes were not compromised on the easy pattern separations compared with the other patients, but they were statistically significantly poorer at the difficult separations.
The results show that operating conditions for SMB and Varicol processes should be selected as a compromise between separation performance and robustness.
Despite prior JAK inhibition, Oct4+/− EpiSCs were able to form occasional colonies when replated in GMEMβ-FCS-LIF, suggesting that, in the presence of reduced Oct4 protein, the stringent separation between ESC and EpiSC states is compromised.
