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The possibility of an even larger clade comprising this last two could also be put forward, consistent with the dispersion along the principal component analysis.
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Squid rhodopsin contains an additional C-terminal domain that might be involved in G protein binding, but it was not structurally characterized as it was necessary to proteolytically remove this domain comprising the last 90 amino acids for crystallization.
This was the era roughly comprising the last third of 2001.
All of these events comprising the last five years of my life are summed up on this record.
Experimental analysis of the mutants shows that the C terminus of the molecule (comprising the last and edge β-strand) is the major contributor to amyloid fibril formation, in good agreement with theoretical predictions.
The previously described "Rab5 binding domain" (R5BD) comprising the last 73 amino acids of rabaptin-5 [20] was obtained by RT-PCR from total HeLa cells mRNA and cloned into the pGEX-6P-1 plasmid.
Surprisingly, the GPS motif is an integral part of the GAIN domain (comprising the last five β-strands).
Guinea pig anti-DRPR antibody was raised against a GST-tagged fragment comprising the last 220 amino acids of the DRPR protein.
Forward primer LL16 was paired with reverse primer LL31 comprising the last 20 nt of the bovine U6 promoter, and all other EGFP scrambled shRNA components.
Universal primer M13 Forward was paired with reverse primer TD134, comprising the last 20 nt of the mouse promoter, and all other EGFP shRNA components.
Together, these results suggest that a region of the Cdx2 transcript comprising the last 97 nt of the ORF is sufficient for localization.
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