Temporally, we found that the Nematostella life cycle comprises two fluorescent phases: 1) a mono-fluorescent phase characterized by the presence of green fluorescence during early polyp stages; and 2) a bi-fluorescent phase starting at the 12-tentacle stage, defined by the appearance of red fluorescence in the oral endoderm.
We describe the SAR of a series of optical imaging agents comprising bulky fluorescent functional groups tethered onto NSAIDs or COXIBs to create dual function fluorescent COX-2 inhibitors.
The control RNA comprised green fluorescent protein (gfp 39, or in experiments requiring luminescent Plasmodium detection, secreted placental alkaline phosphatase (seap 40.
The ensembles are comprised of fluorescent dye (NAHBDS) and boronic acid substituted viologens (BBVs) quenchers/receptors.
To quantify TEVp activity in living cells, we measured Förster resonance energy transfer (FRET) of reporter proteins comprised of fluorescent proteins linked together with a peptide containing a TEVp substrate recognition sequence (TRS).
Interestingly, it could be shown in the study dealing with the dually labeled cysteine cathepsin substrates [Cu] 9 and [Cu] 10 that the introduction of the NIR dye can result in a favorable prolonged circulation compared to the Cu-DOTA-modified analog not comprising a fluorescent dye [ 52].
A mechanochromic composite comprises an excimer-forming fluorescent dye dispersed in a compatible polymeric host.
One such modality, optical imaging, comprises using bioluminescent and fluorescent reporters and probes to repetitively interrogate molecular events and monitor disease progression in animal models.
Our 'basic' polyprotein construct comprised enhanced yellow fluorescent protein (EYFP), 2A, enhanced cyan fluorescent protein (ECFP), a second 2A and puromycin resistance (PAC; plasmid pPDF20).
We have synthesized a fluorescent probe that comprises collagelin, a specific collagen binding peptide, coupled to fluorescent porphyrin that can effectively detect abnormal deposition of collagen in live tissues by emitting fluorescence in the near infra red (NIR) region.
