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Interventions for bradycardia, tachycardia, hypertension and hypotension comprised titration or interruption of study agent, or additional drug therapy.
The most common ICU interventions comprised titration of continuous infusions of IV antihypertensive drugs for uncontrolled hypertension (37/72 patients; 51.4 %), respiratory and airway compromise (27/72; 37.5 %), and management of cerebral edema (15/72; 20.8 %).
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Patients with at least eight seizures during the baseline period then entered a double-blind phase that comprised a 2-week titration period, a 12-week maintenance period, and a 2-week tapering-off period.
Each assay also includes six positive quality controls comprised of a linear titration of in vitro transcribed RNA transcripts and corresponding probes, and eight negative quality controls consisting of probes with no sequence homology to human RNA sequences [ 23].
The study was designed with an 8-week prospective observation phase to establish baseline seizure frequency, which was followed by a double-blind treatment period comprised of a 4-week titration phase, 12-week maintenance phase, and 3-week interval for blinded conversion to open-label treatment or tapering to baseline therapy.
Protective ventilation comprised 8 ml/kg VT and titration of PEEP such that the plateau pressure (Pplat) was 30 cmH2O [ 3].
Positive controls are comprised of a six point linear titration of in vitro transcribed RNA covering an approximately 1000 fold RNA concentration range (0.125 – 128 fM) and corresponding probes [ 29, 30].
Furthermore, such therapies often comprise complex treatment and titration regimens and can increase the risk of hypoglycaemia and undesirable effects such as oedema and weight gain [ 1].
The part of each intervention that was common to both physician groups was comprised of three simulated cases, two of which focused on insulin initiation and titration.
Before use, the two NTA groups of Cy3NTA were charged with 20 nmoles of NiCl2 in water to form a stock solution comprised of 500 µM Cy3NTA and 1 mM NiCl2 used for Cy3NTA titrations in functional assays.
The study comprised three phases: screening and a 0 3-week washout period; a 4-week dose titration period; and a 4-week double blind, placebo-controlled, randomized, maintenance phase.
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