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Conjugation conditions selected for this step comprised the addition of a three-fold molar excess of Df-Bz-NCS to the mAb solution (13 66 nmol mAb), a reaction pH of 9.0, and incubation for 30 min at 37°C.
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These modifications comprise the addition of different type and number of fabric reinforcements in the bonding surface.
A traditional hospital-based optometric service is being compared with an integrated service (comprising the addition of community-based rehabilitation officer input) and with more generic community input (which is non-integrated and is not vision specific).
The MS-based quantification methods usually comprise the addition of defined quantities of isotopically labeled standards to the analyzed sample for the absolute quantification of compounds [ 9, 10].
In the exenatide BID cohort, 74.3% of the first significant treatment changes were discontinuations of initial injectable therapy, with the rest comprising the addition of oral or injectable antidiabetes medication to their exenatide BID regimen.
The latter comprise the addition of methyl, acetyl, and phosphoryl groups or even larger moieties such as binding of ubiquitin or small ubiquitin-like modifier.
The later was based on isoniazid, rifampin, and pyrazinamide from 1994 to 1997, but due to questions of resistance to rifampin, a new scheme of four drugs comprising of the addition of streptomycin or ethambutol to the former emerged.
The antituberculous treatment applied to 39 (44.8%) of the patients from 1994 to 1997 were isoniazid, rifampin, and pyrazinamide while from 1998 to 2005 and due to questions of resistance to rifampin, a new scheme of four drugs comprising of the addition of streptomycin or ethambutol to the former came into use as was commented previously.
Each species was cultivated in 2 × 250ml conical flasks with 100 ml of suitable media: Bolds Basal Medium pH 6.5 (BBM) for eukaryotes and Blue Green-11 Medium pH 7.1 (BG-11) for prokaryotes and a 'Diatom Media' for the diatom (DM), comprised of BBM with the addition of 100μl B vitamins (0.0024% B1B7B12 1:1:1) and 100μl sodium silicate.
Stage 2 can be initiated by the addition of a cocktail comprising HGF, FGF-2/-4, and BMP-2/-4.
This is now termed the ECOG/WHO score (Oken et al, 1982) having been expanded to comprise of six points with the addition of PS 5.
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