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For the transcriptome sampling, seeds at three developing stages (30, 60, 90 DAP) were collected from the same individual, and pods with the same developing stage from two trees comprised of two replicates.
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The Whitehead dataset was comprised of two replicate microarrays hybridized with a spike-in mixture.
Each treatment group was comprised of two replicate tanks, with a volume of 35 L, each containing 8 fish.
Thirty (30) birds were assigned to each treatment which comprised of three replicates of ten birds.
Each genotype comprised of three replicates, each of five seeds.
Each group sample set was comprised of three replicates.
Alternately, cell proliferation was measured by the standard MTT (3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium-bromide; Sigma Aldrich) assay in a 96-well format, comprised of six replicates for each condition.
For the HeLa cell proliferation assay, cells were transiently transfected for 24 h, and then seeded into 96-well plates (6 × 10 cells/well), with each group comprised of six replicates.
The details of the number of reads for each library are shown in Table 1.> -wrap-foot> Each tissue library comprised of three biological replicates.
Participants comprised of two groups.
Each "run" was comprised of four groups of three unpaired technical replicates, one group for each combination of template and primers (reference DNA with reference primers (referenceference DNA with test primers (R/test test DNA with reference primers (T/R') and test DNA with test primers (T/T')), resulting in 12 cycle threshold measurements (Cts) per run.
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