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Although the relevance of these, and all, xenografts to patient tumors has been questioned [5] [7], transplantation of human cells into mice is the only model system that does not expose human cells to an aphysiological ex vivo environment comprised of tissue culture plastic and culture medium and which can recapitulate the cellular heterogeneity of a primary tumor in vivo.
The mammalian organism is comprised of tissue types with varying degrees of self-renewal and regenerative capacity.
However, both ES cells and EpiSCs are competent in multi-lineage differentiation, where injection of these cells into immune-compromised mice results in the development of teratomas comprised of tissue types characteristic of the three germ layers [ 4, 9].
The MM was comprised of tissue culture medium 199 (TCM199) supplemented with 2.5 mM Na-pyruvate, 100 IU/ml penicillin, 100 μg/ml streptomycin, 1 mg/ml estradiol-17β, 10 μg/ml FSH, 10 μg/ml LH, 100 ng/ml EGF, 100 ng/ml FGF, 0.1 mM cysteamine, and 10%% fetal calf serum (FCS) at 39 °C, 6%% CO2.
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The piPSCs could form teratomas when injected into immune-deficient mouse, and the teratomas were comprised of tissues from three germ layers (Fig. 1I).
Organs are comprised of tissues, which are themselves made up of cells.
The Diffusion-Sorption model better explains previously reported difficulties in staining large samples comprised of tissues with high partition coefficients (K d ).
Analyses of TSS from 1193 human samples and 457 mouse samples comprised of tissues, primary cells and cell lines (only one mouse cell line was investigated in FANTOM5) identified 8 TSSs for human FOXG1 and 6 TSSs for mouse Foxg1 (Additional file 2: Table S2).
Many human tissues are comprised of multilayered tissue structures in which spatial organization is essential to provide biological tissue functions.
The breast is comprised of adipose tissue and dense fibroglandular tissue, and women with a high percentage dense breast area (%DBA) or absolute dense breast area (ADBA) measured by mammography are at an increased risk of breast cancer.
The inner high-signal intensity is the true lumen and granulation tissue, and the outer low-signal intensity part is comprised of fibrotic tissue.
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