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In addition to the 12- and 23RSSs flanking each Dβ1 and Dβ2 gene segments, we tested the frequently used Jβ1.1- and Jβ2.5 12RSSs [32]; the Vβ2 23RSS, comprised of genuine heptamer and nonamer consensus motifs; and the Vβ14 23RSS, used previously to define and analyze the B12/23 constraint [16], [20].
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