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Subjects included in our research were females with age comprised in the range of 18 40 years.
Calibration curves using a 4-fold serial dilution of a cDNA pool revealed PCR efficiencies comprised in the range of acceptance (90-110%) and error values < 0.2 (see Table 1).
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Best results are obtained by using the SST k−ω turbulence model, with values of non-dimensional scaled distance to wall y+ comprised in the range 0.2 0.5, including the turbulent dispersion of droplets.
BPCA [ 30]: For the OC, OS and GH2O2 datasets, and for τ comprises in the range 0.5 to 10-15% of missing data, BPCA appears to have one of the lowest RMSE values [see Additional file 2], only bypass by two other approaches.
The total DNA concentration was 41.75 ng μL-1, comprising fragments in the range of 700 9000 bp with an average DNA fragment size of 4.2 kb.
The intra-assay mean precision (repeatability), calculated with the three quality control samples, was 10.8%, the interassay mean precision (reproducibility) was 17.0% and the accuracy was comprised in a range of 100 ± 30% for these three quality-control samples.
The estimation error was comprised in a range of 5 ÷ 20%, generally acceptable for this kind of application.
Calibration curves comprising five concentrations in the range of 0.586-9.375 μg/mL were measured before and after the measurements of enniatin B from B. subtilis extracts (Fig. S1).
It was found that CA values of PS surfaces comprised merely fibers were in the range of 140° 150°.
In an effort to produce bioactive, injectable nanoparticles that carry both TRF and Simvastatin, parenteral lipid nanoemulsions loaded with both compounds were synthesized; nanoemulsions are comprised of nanoscale droplets in the range of 1 100 nm and have many applications in pharmaceutics (reviewed in [ 83]).
To experimentally validate the predicted mutant spots lying within the region of interest, we applied Sanger sequencing on PCR fragments amplified from the six representative SNP candidates numbered 1, 4, 8, 9, 11 and 12 in Fig. 2, comprising the range of graphic patterns and BOD scores.
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