PCR was carried out in an ABI Prism 7700 Sequence Detector (Applied Biosystems, CA) with a thermal profile comprised by 1 cycle at 95°C for 5 minutes; 15 cycles at 95°C for 10 sec, 63°C for 20 sec, and 72°C for 30 sec; and 40 cycles at 95°C for 15 sec, 55°C for 25 sec, 72°C for 35 sec, and 45°C for 30 sec, with fluorescence acquisition at 45°C in the TET and FAM channels.
Absolute quantification using a standard curve (fig. 4 A) revealed that at least 0.2% of PSU haploid genome is comprised by PSUcentSat, corresponding to at least 17,895 copies per haploid genome.
In 2013 the group debuted Submergence, a spectacular installation comprised by 8,604 light bulbs at the Galleri ROM in Oslo, Norway.
A more refined view is that the possible worlds comprised by those propositions are centered at an (ideal doxastic) agent at a given time (Lewis 1979).
The subplots were comprised by surface applications of gypsum at 0, 3, 6, or 9 Mg ha−1.
A large-solid-core fiber design comprised by omitting nine air-holes at the center of square-lattice photonic crystal is investigated for the constant air-hole diameter and for the constant pitch length of air-holes.
Intuitively, we can say that the FTC is the subgraph comprised by those strongly connected components at the top of the hierarchy.
Recent high-sensitivity surveys of diverse environmental samples have revealed that the vast majority of the microbial diversity in a specific habitat is comprised by taxa that are present at very low abundances and the most abundant organisms represent only a fraction of the total diversity [Brazelton 2010].
In contrast to some streptococcal species such as S. pyogenes [106], the proportion of the genome comprised by MGEs is relatively small, at 1.8% of the P1/7 genome, suggesting that the pan-genome of this species is smaller than other streptococci [96], [107], and that there may be some limitations or restrictions to the amount of recent horizontal gene transfer in this species.
The pattern 3 was comprised by genes with lower expression at the M segment, like those encoding methylsterol monooxygenase 1 2 (SP803280_c109581_g5) and cysteine-rich repeat secretory protein 38 (SP803880_c110116_g1).
