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The optimum step change conditions required to achieve a 20-h production run without detectable growth of S. thermophilus comprised a step change to 55 °C, applied for 10 min, with a 60-min interval between step changes.
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A process for synthesizing POSS-polyimide star nanocomposites is reported, comprising a step forming porous-type POSS and subsequent step with polyimide precursor.
Therefore while the present study comprises a step in understanding whether and how security has contributed to the drop in burglary, there is more work to be done.
This mechanism comprises a step of OH− discharge followed by a chemical recombination of the OHads and the OH− ions to give O−ads which is then oxidized.
Reactions were conducted using the following sequence: 1 cycle at 94°C for 2 min; 40 cycles comprising a step at 94°C for 30 s and 1 step at 58°C for 2 s; followed by 40 cycles with 1 step at 94°C for 30 s and 1 step at 56°C for 2 s.
Findings confirm that a set of tools comprising a step-wise process from informing the public in an attractive way, collecting information on public opinion, towards fully participatory approaches such as direct involvement in decision-making is most likely to ensure socially inclusive planning.
An integrated consultation course should comprise a step-by-step teaching strategy with a variety of supervisors' feedback modi, adapted to students' competence.
The antihypertensive drugs were determined using a simple two-step procedure developed which comprised a preconcentration step followed by the differential pulse stripping voltammetric quantification.
Amplification parameters were as follows: 94 °C for 3 min, followed by two cycles, each of which comprised a melting step at 94 °C for 45 s, an annealing step at 48 °C for 45 s, extension at 72 °C for 2.5 min, followed by 28 cycles, each of which comprised a melting step at 94 °C for 45 s, an annealing step at 52 °C for 45 s, extension at 72 °C for 2.5 min, and finally at 72 °C for 10 min.
Briefly, the assays comprised a preamplification step, which combines reverse transcriptase and multiplex target amplification PCR, followed by a probe hybridization step, a probe ligation step and a probe amplification step.
The syringe cleaning procedure comprised a washing step before and after sample injection, using methanol as washing solution.
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